Ipswich will face Skybet League Two side Stevenage in the first round of the Skybet EFL Cup at Portman Road.
Southend will host League One rivals Gillingham, with the two sides also meeting in the league on the weekend prior.
Colchester will be away to Championship side Brighton and Hove Albion.
Other notable ties include five-time winners Aston Villa travelling to Luton Town, Grimsby Town visiting Derby County, and Cambridge hosting Sheffield Wednesday .
The ties will be played on the week commencing August 8th, 2016. The final takes place at Wembley, on Sunday 26th February, 2017.
Carlisle v Port Vale
Rotherham v Morecambe
Accrington v Bradford
Rochdale v Chesterfield
Mansfield v Blackburn
Barnsley v Northampton
Cambridge v Sheff Wed
Derby v Grimsby
Oldham v Wigan
Scunthorpe v Notts County
Doncaster v Nott'm Forest
Burton v Bury
Fleetwood v Leeds Utd
Sheff Utd v Crewe
Shrewsbury v Huddersfield
Preston v Hartlepool
Blackpool v Bolton
South section
Peterborough v Wimbledon
Bristol Rovers v Cardiff
Coventry v Portsmouth
Luton v Aston Villa
Brighton v Colchester
Cheltenham v Charlton
Birmingham v Oxford
Southend v Gillingham
Wolves v Crawley
Leyton Orient v Fulham
Ipswich v Stevenage
Wycombe v Bristol City
Walsall v Yeovil
Exeter v Brentford
QPR v Swindon
Barnet v Millwall
Reading v Plymouth
Newport v MK Dons
Southend will host League One rivals Gillingham, with the two sides also meeting in the league on the weekend prior.
Colchester will be away to Championship side Brighton and Hove Albion.
Other notable ties include five-time winners Aston Villa travelling to Luton Town, Grimsby Town visiting Derby County, and Cambridge hosting Sheffield Wednesday .
The ties will be played on the week commencing August 8th, 2016. The final takes place at Wembley, on Sunday 26th February, 2017.
Full draw:
North sectionCarlisle v Port Vale
Rotherham v Morecambe
Accrington v Bradford
Rochdale v Chesterfield
Mansfield v Blackburn
Barnsley v Northampton
Cambridge v Sheff Wed
Derby v Grimsby
Oldham v Wigan
Scunthorpe v Notts County
Doncaster v Nott'm Forest
Burton v Bury
Fleetwood v Leeds Utd
Sheff Utd v Crewe
Shrewsbury v Huddersfield
Preston v Hartlepool
Blackpool v Bolton
South section
Peterborough v Wimbledon
Bristol Rovers v Cardiff
Coventry v Portsmouth
Luton v Aston Villa
Brighton v Colchester
Cheltenham v Charlton
Birmingham v Oxford
Southend v Gillingham
Wolves v Crawley
Leyton Orient v Fulham
Ipswich v Stevenage
Wycombe v Bristol City
Walsall v Yeovil
Exeter v Brentford
QPR v Swindon
Barnet v Millwall
Reading v Plymouth
Newport v MK Dons