Two goals from Tarique Fosu saw Colchester to victory, but other results meant the U's missed out on the play-offs.
Wins for Blackpool and Carlisle on the closing day meant the U's missed out on a play-off position.
Portsmouth were crowned champions, while Plymouth and Doncaster were also promoted. Luton will face Blackpool, and Exeter meet Carlisle in the play-off games.
Wins for Blackpool and Carlisle on the closing day meant the U's missed out on a play-off position.
Portsmouth were crowned champions, while Plymouth and Doncaster were also promoted. Luton will face Blackpool, and Exeter meet Carlisle in the play-off games.